The content of microand macroelements in the oral fluid at different intensity of dental caries
https://doi.org/10.32885/2220-0975-2022-3-45-53
Abstract
Introduction. Due to the high prevalence of caries, the monitoring of enamel and dentin state remains an actual problem in dentistry and contributes to the development of new methods for diagnosing and predicting a cariogenic situation in the oral cavity. One of these methods is the study of the remineralization and demineralization processes in the oral cavity and of the microelements′ and macroelements′ level in the oral fluid.
The aim of the study is to research the level of macroand microelements in the oral fluid in patients with different intensity of dental caries.
Materials and methods. 13 people aged 21 to 35 were examined, including 10 women and 3 men. Based on the results of the dental examination, 2 groups of subjects were formed: 1st (n=4) healthy individuals and patients with a compensated form of caries; 2st (n=9) patients with decompensated form of caries. The subjects collected oral fluid by spitting into a test tube on an empty stomach, after brushing their teeth and rinsing their mouth three times with distilled water. Three samples were taken from each subject on different days, a total of 39 samples. The content of elements (Na, K, Ca, Mg, Fe, P, Mn) was determined by inductively coupled plasma atomic emission spectrometry.
Results. An increase in the content of most microand macroelements (Na, K, Ca, Mg and P) in the oral fluid was found in patients with decompensated caries compared with the compensated form and healthy people (p<0,05). Perhaps, in the absence of a carious process or compensated caries, the mineral components are concentrated in hard tissues and the remineralization-restoration processes of the tooth mineral structures prevail.
Conclusion. The study of the content of Na, K, Ca, Mg and P in the oral fluid can be used to diagnose and predict the course of the carious process.
About the Authors
O. A. UspenskayaRussian Federation
Olga A. Uspenskaya - Associate Professor, Dr. Sci. (Med.), Head of the Department of Therapeutic Dentistry, eLibrary SPIN: 8616-2393.
Bld. 10/1 sq. Minin and Pozharsky, Nizhny Novgorod, 603005
M. L. Zhdanova
Russian Federation
Maria L. Zhdanova - Associate Professor, Cand. Sci. (Med.), Associate Professor of the Department of Therapeutic Dentistry eLibrary SPIN: 4333-3575.
Bld. 10/1 sq. Minin and Pozharsky, Nizhny Novgorod, 603005
E. L. Kostrova
Russian Federation
Elena L. Kostrova - employee of the center for the collective use of scientific equipment «New materials and resource-saving technologies».
Bld. 10/1 sq. Minin and Pozharsky, Nizhny Novgorod, 603005
Yu. P. Potekhina
Russian Federation
Yulia P. Potekhina - Professor, Dr. Sci. (Med.), Privolzhsky RMU, Professor at the N. Yu. Belenkov Department of Normal Physiology; Institute of Osteopathy (Saint-Petersburg), Deputy Director for Scientific and Methodological Work, eLibrary SPIN: 8160-4052, Scopus Author ID: 55318321700.
Bld. 10/1 sq. Minin and Pozharsky, Nizhny Novgorod, 603005; Bld. 1A ul. Degtyarnaya, Saint-Petersburg, 191024
References
1. Clinical guidelines (treatment protocols) for the diagnosis of dental caries. Approved by Resolution № 15 of the Council of the Association of Public Associations «Dental Association of Russia» dated September 30, 2014, updated on August 02, 2018. Accessed in October 31, 2021 (in russ.). https://oblstom.ru/wp-content/uploads/2018/08/4_karies_8aug2018.pdf
2. Borovsky E. V., Maksimovsky V. S., Maksimovskaya L. N. Therapeutic dentistry. M.: Medicine; 2001; 736 p. (in russ.).
3. Simonova K. K. Biochemical aspects of pathogenesis and prophylaxis of dental caries. Siberian Med. J. 2006; 8: 8–11 (in russ.).
4. Solonenko A. P., Golovanova O. A., Belskaya L. V. Thermodynamic approach to the study of the microelements influence on mineralization in human saliva. Herald Omsk University. 2011; 2: 135–139 (in russ.).
5. Akhmedbeyli R. M., Mammadov F. Yu. Oral saliva parameters of schoolchildren depending on the length of fluorinated iodized salt intake in conditions of biogeochemical deficiency of fluoride and iodide. Med. News. 2016; 4: 61–63 (in russ.).
6. Leontiev V. K., Galiulina M. V., Ganzina I. V., Anisimova I. V., Dashin E. K., Ivanova O. Yu., Panova I. G. Changes in the structural properties of saliva with a change in pH. Stomatology. 1999; 78 (2): 22–24 (in russ.).
7. Coogan M. M., Mackeown J. M., Galpin J. S., Fatti L. P. Microbiological impressions of teeth, saliva and dietary fibre can predict caries activity. J. Dent. 2008; 36 (11): 892–899. https://doi.org/10.1016/j.jdent.2008.07.004
8. Featherstone J. D. Caries prevention and reversal based on the caries balance. Pediat. Dent. 2006; 28: 128–132.
9. Albitskaya Yu. N., Bulkina N. V. Monitoring the extent and prevalence of caries process on the basis of a questionnaire, physical examination of the oral cavity and biochemical methods for saliva. Succ. modern natur. Sci. 2004; 2: 24–25 (in russ.).
10. Pestov A. Yu., Kalashnikova S. A., Kramar V. O. Biophysical parameters of oral fluid in violation of the microflora of the oral cavity. Bull. Volgograd SMU. 2012; 3: 44–46 (in russ.).
11. Razmakhnina E. M., Kiseleva E. A. Characteristics of caries resistance levels depending on the properties of oral liquid in residents of Kemerovo. Med. Kuzbass. 2015; 14 (1): 44–49 (in russ.).
12. Belskaya L. V., Golovanova O. A., Turmanidze V. G., Shukaylo E. S. Prospects for using of saliva analysis in the planning of athletes training regime. Omsk Sci. Bull. 2011; 6 (102): 175–178 (in russ.).
13. Rakhmanov R. S., Alikberov M. Kh., Bogomolova E. S., Gruzdeva A. E. Revisiting the food products development for prevention of dental caries. Med. Newslett. Vyatka. 2019; 2 (62): 50–55 (in russ.).
14. Cheng L., Li J., He L., Zhou X. Natural products and caries prevention. Caries Res. 2015; 49 (Suppl. 1): 38–45. https://doi.org/10.1159/000377734
15. Devyatov I. A., Mashkantseva M. Yu., Ermishina E. Yu., Belokonova N. A., Kalegina S. I. The influence of drinking water quality on the content of calcium and magnesium in saliva // In: Proceedings of the V International Student Scientific Conference «Student Scientific Forum» February 15 — March 03, 2013 (in russ.).
16. Belskaya L. V., Golovanova O. A. Experimental study of the parameters of human saliva as a mineral-forming medium. Bull. Otdelenia nauk o Zemle RAN. 2012; 4: NZ9001 (in russ.).
17. Belskaya L. V., Golovanova O. A. Investigation of the Chemical Composition of Saliva for the Purpose of Diagnostics of Oral Cavity Diseases. Chem. Sustainab. Developm. 2008; 3: 267–272 (in russ.).
18. Domenyuk D. A., Karslieva A. G., Ivancheva E. N., Gilmiyarova F. N., Bykov I. M., Kochkonyan A. S. Relation between hematological indices of calcium-phosphorus metabolism and metabolic parameters in the oral fluid in patients with dentofacial pathology. Kuban Sci. Med. Bull. 2015; 1: 54–65 (in russ.). https://doi.org/10.25207/1608-6228-20151-54-65
19. Lukinykh L. M. Dental caries. Etiology, clinic, treatment, prevention. N/Novgorod: NizhGMA Publishing House; 2004; 186 p. (in russ.).
20. Thompson M., Walsh D. N. Guide to spectrometric analysis with inductively coupled plasma. M.: Nedra; 1988; 174 p. (in russ.).
21. Antonova I., Gorbacheva I., Donskaya O., Titova M. Crystallographic method of investigation of oral liquid of patients with biomineralopathology (literature review). Parodontologiya. 2017; 22 (4): 4–8 (in russ.).
22. Ismail A. I., Hasson H. Fluoride supplements, dental caries and fluorosis: A systematic review. J. Amer. Dent. Ass. 2008; 139 (11): 1457–1468. https://doi.org/10.14219/jada.archive.2008.0071
23. Martynov A. I. Magnesium deficiency conditions in the clinic of internal diseases // In: III International Scientific and Practical Conference of therapists. Kursk, September 08, 2010 (in russ.).
24. Bel′skaja L. V., Golovanova O. A. Laboratory saliva-composition diagnostic technique for oral diseases: Patent of RF № 2367959/20.09.2009(inruss.)].https://new.fips.ru/Archive/PAT/2009FULL/2009.09.20/DOC/RUNWC1/000/000/002/367/959/DOCUMENT.PDF
25. Redinova T. L., Pozdeev A. R. Clinical methods of saliva research in dental caries. Izhevsk: Izhevsk SMI; 1994; 24 p. (in russ.).
26. Turlak I. V. The saliva — the main directions of research of its properties. Modern probl. Sci. Educat. 2020; 4. Accessed in October 31, 2021 (in russ.)]. https://science-education.ru/ru/article/view?id=29934
Review
For citations:
Uspenskaya O.A., Zhdanova M.L., Kostrova E.L., Potekhina Yu.P. The content of microand macroelements in the oral fluid at different intensity of dental caries. Russian Osteopathic Journal. 2022;(3):45-53. (In Russ.) https://doi.org/10.32885/2220-0975-2022-3-45-53